The goalkeeper has sprained his right ankle and will not be travelling for the team’s last game of the season away to Betis.
Guardiola is taking 15 first team players, which include Keita and youngsters Oier, Tello, Montoya and Bartra.
Alexis, meanwhile, is still sidelined and also misses the trip to the Benito Villamarín.
Puyol is out, and has an operation on Saturday, while Alexis is still not fully recovered from his hamstring injury. Longer term absentees Villa, Fontàs and Abidal are also missing.
The side had their last training session before facing Betis on Friday, although future first team manager Tito Vilanova is still away with a virus.
The session started with hearty congratulations for Andrés Iniesta, who is 28 today. (via FCBarcelona.com)
Valdés, Alves, Piqué, Cesc, Xavi, Iniesta, Messi, Thiago, Mascherano, Keita, Sergio, Pedro, Afellay, Adriano, Cuenca, Oier, Tello, Bartra and Montoya.